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Tissue Homogenization
Ultrasonic homogenizers make use of the ultrasonic waves generated by a piezoelectric crystal. When a certain voltage is applied to the crystal, the ultrasonic waves are transmitted through water or air, leading to cavitation which in turn leads to the generation of forces on nearby surfaces. These powerful forces homogenize tissues, breaking apart connective tissues , lysing cells, and releasing cellular contents which may then be purified and utilized in a wide range of downstream analyses.
At Qsonica, we offer a wide variety of Sonicators that are reliable, powerful, and proven. When you need a trustworthy instrument for tissue homogenization, do what tens of thousands of scientists have done for half a century: rely on a Sonicator.
Product applications
- DNA / RNA extraction
- Protein extraction / proteomics
- Organelle isolation
- Small molecule extraction / metabolomics
- Pharmaceutical compound extraction for ADME studies
- Forensics / toxicology
Videos
Tissue Homogenization Publications and Protocols
Protocols
Publications
- Structural basis for a complex I mutation that blocks pathological ROS production
- Characterization of tau prion seeding activity and strains from formaldehyde -fixed tissue
- Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS) spectra of salivary glands carcinoma, tumor and healthy tissues and their homogenates analyzed by chemometry: Towards development of the novel tool for clinical diagnosis
- A rapid and convenient method for measuring the fractional rate of protein synthesis in ectothermic animal tissues using a stable isotope tracer
Tips & Info for Tissue Homogenization
- Sonicators are for research use only.
- Sonicators are suitable for use with soft tissues. Hard tissues, such as bone or wood, are not suitable for use with ultrasonic homogenizers and may damage the horn.
- Very tough, fibrous tissue such as ligament may not be homogenized efficiently using an ultrasonic homogenizer. Should you wish to process such tissue, you may wish to pre-treat with collagenase or a similar protease to soften the tissue.
- All tissue being processed must be fully immersed in liquid, and the tip of the horn must remain below the liquid surface at all times.
- Do not over-homogenize! Running for longer than necessary may degrade RNA, denature proteins, and shear DNA.
- For general best practices, see Best Practices for Ultrasonic Homogenization.
