Q800 Sonicator
High throughput DNA & Chromatin shearing
The Q800R3 Sonicator is a chromatin / DNA shearing system which provides scientists with a reliable, easy-to-use means of achieving narrow DNA fragment bands at a cost which is thousands of dollars less than competing systems. The second generation Q800R3 improves upon the design with enhanced usability and quieter operation than its predecessor. Get the DNA shearing you need with the confidence and quality expected from the Sonicator brand.
Samples can be sheared to a range of fragment sizes (150bp – 3kb). The system is compatible with commercially available sample tubes and is capable of processing up to 18 samples at one time. Multiple tube rack options can accommodate 50ul - 1ml sample volumes using a variety of standard sample tubes.
The Q800R3 is a complete package including a chiller to control temperature, digital operating system with programmable memory, high intensity ultrasonics and sound reducing enclosure.
Q800R3 SYSTEM HIGHLIGHTS:
- High throughput
- Uses standard sample tubes
- Two year warranty
PART NO. Q800R3 INCLUDES:
- Generator
- Converter
- Ultrasonic Horn
- Rotation System
- Chiller
- Cables
- Tubing
- 3 tube rack options
- 2-year warranty
Chromatin Shearing - Sample Protocols
| Mammalian Chromatin | Yeast Chromatin | C. elegans Chromatin | |
| Cell Type: | HEK 293T (2) 10cm dishes 70-80% confluent | Wild type S. pombe cells grown to an OD of 1.3-1.5 in YEA. | C. elegans culture; Nuclear extract 1mg/ml |
| Total Sample Volume: | 300ul | 300ul | 500ul |
| Fixation Time: | 1% Formaldehyde, 13 min | 1% Formaldehyde, 15 min | 2% Formaldehyde, 20 min |
| Sonicator Amplitude Setting: | 70% | 100% | 70% |
| Sonication Pulse Rate: | 15 seconds On, 45 seconds Off | 20 seconds On, 40 seconds Off | 30 seconds On, 30 seconds Off |
| Total Sonication On Time: | 30 min | 30 min | 20 min |
DNA Shearing - Sample Protocols
| Bacterial Genomic DNA | Human Skeletal Myoblast Genomic DNA | |
| Cell Type / Concentration: | E.coli / 250ng | LHCN-M2 Skeletal Myoblast / 200ng |
| Sonication Pulse Rate: | 15 seconds On, 15 seconds Off | 15 seconds On, 15 seconds Off |
| Total Sample Volume: | 200ul in 0.5ml tubes | 200ul in 0.5ml tubes |
| Total Sonication On Time: | As indicated below | As indicated below |
| Sonicator Amplitude Setting: | 20% | 20% |
- Broad Institute
- Sample Prep Protocol
- Tissue Protocol
- Human Cell Line ChIP Protocol
- Mammalian Line ChIP Protocol
- C. elegans ChIP Protocol
- Yeast ChIP Protocol
- Yeast and Mammalian Chromatin Protocol
- 293T ChIP Protocol #1
- 293T ChIP Protocol #2
- T Cells ChIP Protocol
- HCT116 ChIP Protocol
- Jurkats, HEK293 Protocol
- CH12 ChIP Protocol
| Power Rating | 750 Watts |
| Frequency | 20 kHz |
| Programmable Timer | 1 second to 10 Hours |
| Voltage | 110V, 50/60Hz |
| Generator Dimensions | 8" W x 15.25" L x 8.5" H |
| Enclosure Dimensions | 11.5" W x 12" L x 20" H |
| Chiller Dimensions | 13" W x 11" L x 13" H |
| * | 220V not available online, contact us for local distributor. |
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