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Sonication is effective for many applications including:
- Protein Purification
- Degassing
- PMCA
- Sonochemistry
- Liposomes
- Crystallization
- Emulsification
- Extraction
- Transesterification
- Solubilize compounds
- Virus isolation
- Vaccine manufacturing
Virus Research and Ultrasonics
Sonication is a reproducible established method for sample preparation when lysing cells for the release of virus, viral proteins, DNA and RNA.
Virus related applications:
- Cell lysis to extract viruses
- DNA and RNA fragmentation
- Disperse virus clusters
Cup Horn
Sonicators can be used for fast, reproducible sample preparation to extract viruses from tissue and cell culture prior to downstream analyzation of proteins, nucleic acids, etc. Ultrasonic processing can also be used to shear DNA and RNA for sequencing.
Cup Horns are ideal for sterile or pathogenic sample processing and enables multiple tubes to be processed at one time. Samples can be processed in sealed tubes or vials eliminating aerosols and cross contamination.
Vaccine Production and Drug Formulation with Ultrasonics
Sonicators are used in vaccine production to create nano-sized drug delivery platforms. Systems can be sized for use in R&D, lab scale as well as industrial scale production.
Sonication can be used to:
- Lyse virus or bacterial cells to release proteins, antigens, DNA/RNA, etc.
- Destroy or inactivate viruses and bacteria.
- Prepare nano-sized drug delivery systems.
- Increase bioavailability by forming nanoparticles, nanoemulsions and liposomes.
- Create homogeneous suspensions with ultrasonic dispersion and deagglomeration.
Production Scale Sonicator:
Videos
Publications and Protocols
Frequently Asked Questions
Tip Depth / Foaming Issue - Probes/tips must be submerged properly. If the tip is not submerged enough the sample will foam or bubble. If the tip is too deep it will not circulate the sample effectively. Both conditions will end up with poor results. Foaming often occurs with samples volumes below 1ml. Foaming can also be caused when the amplitude setting is too high.
Visit the FAQ section for answers to more questions about sonication.
Other Applications Publications and Protocols
Protocols
- Lipid Preparation Protocol
- ASTM G32-10 – Standard Test Method for Cavitation Erosion Using Vibratory Apparatus
- Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization
- Degassing Testimonial
- Elution of antibodies from tips (Mitra Tips from Neoteryx)
Publications
Q700
Nanoemulsion: an advanced mode of drug delivery system
Jaiswal, Manjit et al. 3 Biotech, 2015 Apr; 5 (2): 123–127.
Ultrasonic Aggregate Breakdown of an Oxisol as Affected by Cavitation Intensity
Ribeiro, B. T., et al., Journal Communications in Soil Science and Plant Analysis, 2017, Vol. 48, No.7, pp. 818-824, DOI: 10.1080/00103624.2017.1299170
Effect of Temperature and Sonication on the Extraction of Gallic Acid from Labisia Pumila (Kacip Fatimah)
Noor Adilah Md et al., ARPN Journal of Engineering and Applied Sciences, Volume 11, Number 4, February 2016, Salehan
Exosomes as Drug Delivery Vehicles for Parkinson's Disease Therapy
Matthew J. Haney et. al., Journal Control Release, 10; 207: 18-30, 2015, June
Human prion protein sequence elements impede cross-species chronic wasting desease transmission
Kurt et. al., Journal of Clinical Investigation, Volume 125, Number 4, April 2015, pp1485-1496
Crystallization and preliminary X-ray diffraction analysis of the organophosphorus hydrolase OPHC2 from Pseudomonas pseudoalcaligenes
Gotthard, et.al., Acta Cryst. (2013). F69, 73-76
Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds
Freitas, et.al., Molecules 2014, 19, 5999-6008
Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells
L. van de Hoef et.al., PLOS ONE, Volume 8, Issue 2, February 2013
Quaternary Structure of Pathological Prion Protein as a Determining Factor of Strain-Specific Prion Replication Dynamics
Laferriere et.al., PLOS ONE, Volume 9, Issue 10, October 2013
Red-Backed Vole Brain Promotes Highly Efficient In Vitro Amplification of Abnormal Prion Protein from Macaque and Human Brains Infected with Variant Creutzfeldt-Jakob Disease Agent
Nemecek et.al., PLOS ONE, Volume 8, Issue 10, October 2013
Q55
Rapid detection of bacteriophages in starter culture using water-in-oil-in-water emulsion microdroplets
Lipscomb et. al., Applied Microbiology and Biotechnology, August 2014, DOI 10.1007/s00253-014-6018-7
Discontinued Models (e.g. S-4000, S-3000)
Trans-species amplification of PrPCWD and correlation with rigid loop 170N
Kurt et. al., Virology, March 2009, doi:10.1016/j.virol.2009.02.025
A Proposed Mechanism for the Promotion of Prion Conversion Involving a Strictly Conserved Tyrosine Residue in the β2-α2 Loop of PrPC*
Kurt et. al., Journal of Biological Chemistry, Volume 289, No. 15, pp. 10660-10667, April 2014
An overview of Ultrasonically assisted extraction of bioactive principles from herbs
Vinatoru et. al., Ultrasonics Sonochemistry, 8, 2001, 303-313
De Novo Growth Zone Formation from Fission Yeast Spheroplasts
Kelly et.al., PLoS ONE 6(12): e27977. (2011); doi:10.1371/journal.pone.0027977Acta Cryst. (2013). F69, 73-76
Cyanobacterial biomass as carbohydrate and nutrient feedstock for bioethanol Production by yeast fermentation
Mollers et. al., Biotechnology for Biofuels, Volume 7, No. 64, 2014
